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Genome-wide core sets of SNP markers and Fluidigm assays for rapid and effective genotypic identification of Korean cultivars of lettuce (Lactuca sativa L.)

Author
Park J.S.,Kang M.Y.,Shim E.J.,Oh J.H.,Seo K.I.,Kim K.S.,Sim S.C.,Chung S.M.,Park Y.,Lee G.P.,Lee W.S.,Kim M.,Jung J.K.
Journal Title
Horticulture Research
Publication Year
2022
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Summary

This study aimed to establish an effective and high-throughput cultivar identification system for lettuce by developing core SNP marker sets through genotyping by sequencing (GBS), identifying 17,877 high-quality SNPs. The developed SNP marker sets were validated using the Fluidigm platform, and phylogenetic analysis successfully discriminated individual cultivars. These core SNP markers will support the construction of a DNA database useful for cultivar identification, purity testing, and DUS testing.

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